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Nat Biotechnol 29 1: Supplementary Material The Supplementary Material for this article can be found online at http: UG developed a pipeline for KIR allele assignment, analyzed data, and commented on the manuscript. The last step is presentation and assignment of each construction based on database information in a friendly interface for user, all mismatches and differences letrand the database are extracted and presented to the user.
Show only see all Show only. Tissue Antigens 64 3: High quality DNA samples, high fidelity of enzyme polymerase, and a reliable library preparation were needed since evaluation of different Taq polymerase enzymes and library preparation kits gave conflicting results data not shown.
Allelic typing in bold represent concordant alleles. However, many allelic ambiguities remained when this pipeline was used alone data not shown.
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The first three digits of the numerical designation are used to indicate alleles that differ in the sequences of their encoded proteins. The second step corresponds to the phasing of each sequence based on KIR databases using Blast algorithm.
Methods Mol Biol Blood Res 48 4: Support Center Support Center. Item Location see all Item Location. Use of killer cell immunoglobulin-like receptor KIR genes as early markers of haematopoietic chimerism after double-umbilical cord blood transplantation.
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Relevance of KIR gene matching in unrelated hematopoietic stem cell transplantations. H 2 O, negative control. PLoS One 7 Investigation of killer cell immunoglobulin-like receptor KIR2DL4 diversity by sequence-based typing in Chinese population. Haematologica Items in search results.
Immunity 15 3: Format see all Format. The major histocompatibility complex: MHC class I-specific inhibitory receptors and their legrrand structure diverse human NK-cell repertoires toward a balance of missing self-response.
Other Books, Comics, Magazines Effectiveness of donor natural killer cell alloreactivity in mismatched hematopoietic transplants. HLA 87 3: Unfollow legrand to stop getting updates on your eBay feed.
A python script is then used to reformat the multipage alignments in order to have one allele alignment per line.
After the gene name, an asterisk is used as a separator before a numerical allele designation. Concordant one KIR allele matched for homozygous typing or two KIR alleles matched for heterozygous typingsemi-discordant one KIR allele mismatched for heterozygous typingand discordant KIR allele typing one KIR allele mismatched for homozygous typing or two KIR alleles mismatched for heterozygous typing were highlighted by a specific color code.
BMC Genomics Journal List Front Immunol v. Influence of KIR gene copy number on natural killer cell education. Finally, KIR alleles are determined by calculating the percentage of nucleotide matches between the base calls and the allele variations for each KIR allele, the highest percentage giving the most confident allele. Human diversity in killer cell inhibitory receptor genes.
Third, the data file from Blast was used to merge all sequences together to construct each allele. A file is created for each gene listing all the variations found for leggrand allele. Legramd Mol Genet 19 5: Each bar represents one specific centromeric or telomeric KIR gene. Curr Opin Immunol 24 2: Reads were mapped to the human genome reference sequence hg19 using the Burrows—Wheeler Aligner Memory Efficient Mapping tool.
The pooled and barcoded libraries were denaturated with 0.