API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
After the addition of Nitrate reagents A and B: Reactions obtained using the Microbact System may differ from published results using other substrate formulations. The 12A strip may be used alone for identification of oxidase-negative, nitrate-positive glucose fermenters comprising 15 genera and may be useful for screening pathogenic Enterobacteriaceae from enteric and urine specimens or identification 200e other common isolates.
20f of indolepyruvate by deamination of tryptophan. Evaluate 15 to 30 minutes after the addition of reagents. Record the results under the appropriate heading on the report form. Bromothymol blue indicates formation of the specific amine cadaverine.
This is a negative reaction. Each strip consists of 12 different biochemical substrates. Mix thoroughly to prepare a homogeneous suspension. The results of tests for oxidase, nitrate reduction and motility are included as part of the reaction pattern.
This code is entered into the computer package. Used materials should be autoclaved, incinerated, or immersed in germicide before disposal. Motility and DNase tests are recommended for further speciation of this group.
Yellow-green is indicative of a negative result. The reactions are evaluated as positive or negative by comparing them with the colour chart. The following organisms are recommended for independent laboratory assessment.
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For aid in interpreting reactions, refer to the Table of Reactions. Warning and precautions 1.
H 2 S is produced from thiosulphate. Biochemical Characterisics of Enterotoxigenic Aeromonas sp. Hydrolysis of gelatin is indicated by dispersal of the black particles throughout the well.
Kit presentation Each kit galegie the following: The inclusion 20s a 12B strip is strongly advised. Evaluate within 2 minutes of the addition of the reagent. Identification of Bacteria by Computer: Yellow-green – Negative Blue – Positive. When Actinobacillus or Pasteurella spp. Some bacterial strains may have atypical biochemical reactions due to unusual nutritional requirements or mutations and may be difficult to identify.
Citrate utilization citrate is the only source of carbon. Indole Kovacs reagent forms a pink-red complex with indole. Record all positive results.
Green reactions occurring at 48 hours should be interpreted as negative. One drop of Nitrate reagent A and 1 drop of Nitrate reagent B is added to the well. The sum of these indices in each group of three reactions forms the code number.
MBA Microbact 12E 20 pouches, identifications. Preparation of inoculum Pick isolated colonies from an hour culture and emulsify in 2. When Actinobacillus or Pasteurella sp.
Material Safety Data Sheet. Bromothymol blue indicator changes from blue to yellow when the carbohydrate is utilised to form acid. The 12 substrates contained in the 12A strips are available in a solid microplate format, referred to as 12E. Set up proceedure Isolation An hour pure culture of the organism to be identified falerie be obtained.
Inoculation The wells of individual substrate sets spi be exposed by cutting the end tag of the sealing strip and slowly peeling it back. Microbact Biochemical Identification Kits. The pH shift indicated by bromothymol blue caused by formation of the specific amine putrescine is greater than that caused by lysine decarboxylation.
Indole positive organisms may produce a brown colour. These strips are intended for in vitro use only; for use by qualified laboratory personnel using aseptic techniques and established precautions against microbiological hazards. Miscellaneous Gram-negative bacilli – Weakly positive reactions are recorded as negative results. The 12B strip can be used in conjunction with the 12A strip for the identification of oxidase-positive, nitrate-negative, and glucose-nonfermenters MGNB as well as the Enterobacteriaceae.