EJEMPLOS DE OXIDORREDUCTASAS PDF

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Este tipo de empaques se ha utilizado para mantener las propiedades sensoriales de quesos y mayonesa durante su almacenamiento BANKAR et al. A recombinant host cell is a bacterial cell, insect, plant or mammalian and transformed or transfected with an expression vector according to claim 4. Por regla general, estas enzimas dependen de cofactores.

By the term “halogen” means an element of the series fluorine, chlorine, bromine or iodine.

Los cosustratos adecuados de la formiato Suitable cosubstrates of formate. The reaction oxdorreductasas amounted to 1 hour to 48 hours, in particular from 2 hours to 24 hours.

Amplification of full-length DNA encoding the protein from genomic DNA with restriction and subsequent ligation into the expression vector was performed as described in Example 3. Reaction mixture for the determination of ee value: Enzymatic hydrolysis of 3,3-diethyl[ 4-carboxy phenoxy]azetidinone esters. Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. In this respect it is recorded particularly in the pharmaceutical industry a growing need for chiral compounds and thus chiral synthesis technologies, since in the oxidorreductassa and only be used as drugs oxidorreeuctasas compounds.

The reaction conditions are essentially the same as in the aforementioned method for the enantiospecific reduction of the ketone compound of formula I.

The lysate obtained was then used to dilute manner corresponding measurements. Para el aislamiento de las oxidorreductasas microbianas dependientes de NAD P H se cultivaron los microorganismos tal como se ha descrito en el ejemplo 1. Los modelos de sitio activo para las enzimas que contienen molibdopterina, se encuentran basados en una clase de ligandos conocidos como ditiolenos.

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The extract obtained cells can be used either directly or be purified further. Por lo tanto, las molibdopterinas pueden formar complejos tanto con molibdeno como con tungsteno, por ejemplo, en las bacterias.

Preferably procedures are performed in accordance with the invention however without additional dehydrogenase of this type, ie does not occur coenzyme regeneration coupled to the substrate.

As different substrates were used ketones such as 2-butanones, 2-octanone, 4-chloroacetoacetate, acetophenone or acid ethyloxophenylbutyric. The resulting nucleic acid served as a sjemplos for the polymerase chain reaction PCR with degenerate primers. Los restos arilo C6-C14 son por ejemplo fenilo, naftilo, por ejemplo 1-naftilo, 2-naftilo, bifenililo, por ejemplo 2-bifenililo, 3bifenililo y 4-bifenililo, antrilo o fluorenilo.

Suitable cosubstrates for these alcoholdeshidrogenasas are the already mentioned secondary alcohols such as ethanol, 2-propanol isopropanol2-butanol, 2-pentanol, 4-methylpentanol, 2-octanol or cyclohexanol.

Examples of heteroatoms N, O and S. Practical Guide for the Food Industry. The recombinant protein expression ds induced by adding isopropylthiogalactoside Oxidorreductassa at a concentration of 0.

Enzimas: aceleradores de reacciones químicas en las células y en la industria

The process according to the invention is performed for example in a closed reaction vessel made of glass or metal. For this, the components are transferred individually into the reaction vessel and stirred under an atmosphere of oxidorreducttasas example nitrogen or air. The substrate is distributed correspondingly to its solubility between the organic and aqueous phase. Compositions and methods for producing stereoisomerically pure statins and synthetic intermediates therefor.

Ketoreductase polypeptides for the stereoselective production of 4s -3[ 5s -5 4-fluorophenyl hydroxypentanoyl]phenyl-1,3-oxazolidinone. By the term aryl residues of aromatic hydrocarbon it is meant having 6 to 14 carbon atoms in the ring. The two liquid phases are preferably mixed mechanically so generated therebetween a large area.

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Examples Ry oxidoreductases and dehydrogenase S-specific suitable high enantioselectivity: The present invention relates to a process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound in the ketonic compound is reduced with an oxidoreductase.

New ketonic ester reductases, its preparation and use for enzymatic redox reactions. American Association of Cereal Chemists,pp. Biphenylyl residues, naphthyl residues and, in particular phenyl radicals are preferred aryl radicals. Los anticuerpos pueden ser monoclonales o policlonales.

Sep de ; 62 4: Cloning and overexpression of the old yellow enzyme gene of Candida macedoniensis, and its application to the production of a chiral compound. El segmento de ADN que codifica la respectiva oxidorreductasa se amplifica con ayuda de polimerasa Platinum pfx Invitrogen.

To determine the pH optimum for the oxidation reaction is used as cofactor NAD P and as substrate 2-propanol or 2-octanol. Tabla 4 table 4. The supernatant lysate obtained after centrifugation of 2 min at rpm was used in the next activity selection and for determining the enantiomeric excess ee value.

Process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound in the ketonic compound is reduced in the presence of a cofactor with an oxidoreductase, wherein.

The invention further relates to an expression vector is a bacterial cell, insect, plant or mammalian and containing a nucleic sjemplos sequence encoding the carbonilrreductasas according to SEQ ID NO: Furthermore, these processes are characterized by side reactions often and insufficient enantiomeric excesses. By the term “C0” means a covalent bond.

Oxjdorreductasas prepararon los tampones mencionados en la tabla 4. Amplification and subsequent reamplification of specific cDNA results in a fragment that binds to the analysis in a pCR2.