• No Comments

Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.

Author: Dogar Groktilar
Country: Bangladesh
Language: English (Spanish)
Genre: Science
Published (Last): 15 May 2008
Pages: 404
PDF File Size: 20.74 Mb
ePub File Size: 9.72 Mb
ISBN: 203-5-77807-373-3
Downloads: 47536
Price: Free* [*Free Regsitration Required]
Uploader: Moogushicage

Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. Agarose gel electrophoresis was used to detect PCR products. The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products.

Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR. Primers sequences, genome positions and the size of PCR products are shown in Table 1. This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract.

Published online Sep 1. Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders Finally, nucleic acids from tissue homogenates samples derived from seven healthy pigs, and a non infected PK cell line were also tested showing no positive products data not shown.

Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders.

Doença de Aujeszky – Wikipédia, a enciclopédia livre

Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown.


Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine.

Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided. Open in a separate window. The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived from seven healthy pigs.

All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License. The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ transplantation 7 and to detect viral infection in a wide spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8. Seven virus-negative tissues samples from clinically healthy animals were also included.

Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 The annealing temperature and number of cycles were determined experimentally. Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome.

In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated. This was possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency. Iowa State University Press; Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR.

Journal List Braz J Microbiol v.

The viral agent following a aujeszkky replication can establish latent infection and develops a latency-reactivation infection which allows its perpetuation in pig populations 1012 Can J Com Med. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis.


Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.

The assay proved to be very sensitive due to as little as 1. The effect of dosna and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.

However, this method is time-consuming and false negative results may occur in submissions from latently infected animals The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome Moreover, the viral genomes of a related herpesvirus and other DNA genome porcine viruses as follow: Potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine.

The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected. Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6.

Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed. Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases PCR experiments were performed on serial ten-fold dilutions of a viral suspension of PRV isolate V with a titer of 10 6.

Doença de Aujeszky

The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I. This region was highly conserved for all reported genomes as shown by aligning of these sequences.