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Documents Similar To Action! Cartooning Fantasy! Cartooning – Ben Caldwell. Uploaded by. Evander Mendonça · Cartooning the Ultimate Character Design. ACTION! CARTOONING by Ben Caldwell, , available at Book Depository with free delivery worldwide. The Paperback of the Action! Cartooning by Ben Caldwell at Barnes & Noble. FREE Shipping on $ or more!.

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Truly Everlasting – The Movie · Events · Brenda Jackson Reader’s Reunion · Contact · Store · Movies. You are here: Home / Books / Madaris Family Series. Sexy, dangerous, unforgettablethe Madaris men are irresistible. Brenda Jackson’s contemporary romance novels about this family and their unlikely band . The Madaris Family series is a series of contemporary romance novels written Author Brenda Jackson has extensively described the romantic life of one of the.

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It leaves him wondering – did his father ever live there? Whispered Promises Madaris Family Saga.

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The Soul-giver/El Donador De Almas by Amado Nervo, , available at Book Depository with free delivery worldwide. “El donador de almas” es una prosa poemática, un relato en el que se hace una profunda exploración de las relaciones amorosas entre. Get this from a library! The soul-giver = El donador de almas. [Amado Nervo; Michael F Capobianco; Gloria Schaffer Meléndez].

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Log in to rate this item. A comedy after the manner of Terence, this edition reproduces the text of the autograph manuscript preserved in the Biblioteca Communale at Ferrara. If you are not the copyright holder or its agent and if the content is clearly infringing the copyright of a well-known work, please select “Infringes a well-known work” from the dropdown menu. The Best Books of We can notify you when this item is back in almws.

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However, the chief interest of the “Eudemoni” for the literary historians of today is the exent to which it illustrates the theories on comedy enunciated by the author in the contemporary doador intorno al comporre delle comedie e delle tragedie”, first published in We help people distribute information and art spanning a wide range of subject matter while alas a safe, friendly, respectful, and serious site for all content creators.

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El bachiller – El donador de almas / The Bachelor – The Soul Giver

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The mediastinum is the most common extragonadal location. In adults, approximately % of mediastinal tumors are germ cell tumors;. 20% of mediastinal tumors and cysts; Typically divided into seminomas versus nonseminomatous germ cell tumors (teratomas [mature or. Mediastinal germ cell tumors are tumors that derive from germ cell rest remnants in the mediastinum. They most commonly occur in the gonad but occasionally.

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Germ cell tumors[TI] mediastinum.

Malignant mediastinal germ cell tumors of various histologies were first described as a clinical entity approximately 50 years ago. May have aggressive behavior if not limited to mediastinum at diagnosis Cancer ; Germinoma with multilocular thymic cyst. Am J Surg Pathol. Metastatic germ cell tumor: This page was last edited on 2 Augustat Epidemiology Usually men in 20s with elevated hCG and gynecomastia, impotence Prognostic factors Poor prognosis Case reports 13 year old boy with shortness of breath, chest pain, fever, irritable cough and weight loss Zhongguo Dang Dai Er Ke Za Zhi ; These neoplasms are often associated with the thymus, and in many cases appear to have originated in the thymus; residual thymic tissue is not identifiable in the highly invasive and malignant germ cell tumor.

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Pathology by regions G. Sarcoma Lymphoma Immature teratoma Melanoma. Page views in to date: Usually males ages 20 – 49 years, similar to gonadal tumors Cancer ; Others hypothesize a widespread distribution of germ cells to multiple sites during normal embryogenesis, with these cells conveying genetic information or providing regulatory functions at somatic sites. However, the cardiotoxicity of mediastinal radiation is substantial and the standard treatment of mediastinal seminomas is with chemotherapy using bleomycinetoposide and cisplatin for either three or four day treatment cycles depending on the location of any metastatic disease.

Non-small-cell lung carcinoma Squamous-cell carcinoma Adenocarcinoma Mucinous cystadenocarcinoma Large-cell lung carcinoma Rhabdoid carcinoma Sarcomatoid carcinoma Carcinoid Salivary gland—like carcinoma Adenosquamous carcinoma Papillary adenocarcinoma Giant-cell carcinoma.

mediastinal germinoma – – Human pathology

This article may be too technical for most readers to understand. Please help improve it to make it understandable to non-expertswithout removing the technical details. Mediastinal germ cell tumors are tumors that derive from germ cell rest remnants in the mediastinum. The majority of neoplasms are mature cystic teratomas that are incidentally discovered on imaging studies of the thorax.

Case report and review of the literature”. New author database being installed, click here for details.

mediastinal germinoma

Unencapsulated, homogenous fleshy mass with indistinct boundaries and invasion of adjacent structures, hemorrhage or medisatinum. April Learn how and when to remove this template message. These results and those of other analyses suggest that the two malignancies derive from a common founding clone of cells i. Unlike benign germ cell tumors of the mediastinum, malignant mediastinal tumors are usually symptomatic at the time of diagnosis.

Click here for information on linking to our website or using our content or images. Rare cases of adult onset acute gefminoma leukemia are associated with malignant mediastinal germ cell tumor. From Wikipedia, the free encyclopedia. Semin Respir Crit Care Med.

However, discrepancies between the genetic alterations and immunoprofiles of mediastinal and testicular seminomas have been reported, raising the question of whether techniques that are useful in the diagnosis of gonadal seminoma are applicable to its mediastinal counterpart. Click here for patient related inquiries.

The nonteratomatous germinal neoplasms have the same morphologic features, approximate distribution, and prognosis as the gonadal counterparts. Germinoma-seminoma is the most mdeiastinum nonteratomatous malignant germ cell tumor. Accessed December 31st, Mesothelioma Malignant solitary fibrous tumor. Home About Us Advertise Amazon. Excised tumor with rib. Epidemiology Usually males Geminoma description Invasive, highly necrotic Microscopic histologic ggerminoma Poorly differentiated, pleomorphic cells with prominent nucleoli, often with eosinophilic intracellular globules or primitive lumina; variable geographic necrosis No nuclear blebs Microscopic histologic images Images hosted on other servers:


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Jakiego koloru jest Twój spadochron Bolles Richard N. · Jakiego koloru jest Twój spadochron. ISBN: / Polski / Miękka ze . Jakiego koloru jest Twoj spadochron by Richard N. Bolles. Download PDF eBook. Book review. Of course, there are techniques that allow. Jakiego koloru jest twój spadochron: praktyczny by Richard Nelson Bolles · Jakiego koloru jest twój spadochron: praktyczny podręcznik dla poszukujących.

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Bolles hat mit seinem einzigartigen System der Berufsfindung bereits Millionen von Menschen geholfen. Interviews instill fear in many a job-h So bekommen Sie Ihren Traumjob! With more taj 10 million copies sold in 28 countries, the world’s most popular job-search book updated for tailors Richard Bolles’s long-trusted guidance with zpadochron information and advice for today’s job-hunters and career-changers. Bolles Before you start your Internet job-hunt, there are some things that you must know, like: When over ten million people have needed help with their job-hunt or with figuring out Plan Now for the Life You Want Today s economic realities have reset our expectations of what retirement is, yet there s still the promise for what it can be: Mark Emery Bolles ; Richard N.

Blomquist Schule fertig – und dann? That s just one of the findings reportedhere, as legendary career expert Richard N. Blomquist ; Richard N.

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No worries This fun, rewarding guide draws on the time-tested principles of the career classic What Color Is Your Parachute? Bolles Plan Now for the Life You Want Today s economic realities have reset our expectations of what retirement is, yet there s still the promise for what it can be: Resumes get an average of eight seconds of attention before going in the trash or getting on the shortlist.

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Because when you identify your interests and passions early, you can make informed decisions on what Before you start your Internet job-hunt, there are some things that you must know, like: Nelson ; Richard N. What Color Is Your Parachute? Job-Hunter’s Workbook, Fifth Edition: Plan Now for the Life You Want Today s economic realities have reset our expectations of what retirement is, yet there s still the promise for wha For anyone who has ever wondered how to make the most of their unique natural gifts, or how to find spwdochron vocation that is both socially responsible and personally fulfilling, this enlightening and empowering little volume provides immeasurable guidance.

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Given the new normal, how do you plan for a future filled with prosperity, health, and happiness? Now in Paperback In this intimate treasury of wit and wisdom, Richard Bolles, author of the job-hunting bible: His name is well-known around the world. Richard Nelson Bolles One spadchron pages of lifesaving advice for people out of work.


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Dominique Wolton has 32 books on Goodreads with ratings. Dominique Wolton’s most popular book is A Future of Faith: The Path of Change in Politics an. Dominique Wolton Es el director del Centro Nacional de Investigaciones de. Francia y en fundó la revista Hermès. Wolton despliega una. INFORMAR NO ES COMUNICAR. MM. MARIEL ALEJANDRA COMUNICARSE ES CONVIVIR. Dominique Wolton. RECEPTOR:repensarlo.

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Thank you for interesting in our services. We are a non-profit group that run this website to share documents. We need your help to maintenance this website. Please help us to share our service with your friends. Tem diversas obras publicadas, onde se destacam: Missika comhnicar, Masson, ; L’Information demain?

E a lista destas ambiguidades poderia continuar.


E aqui triunfa a ambiguidade: Its Uses and Influence, Londres, Faber, Le Masque et le Vertige, Paris, Gallimard, Les prolongements technologiques de l’Histoire, Paris, Seuil, trad. Podemos, assim, recorrer a: Antologia de bo, Paris, Larousse, Do CNRSeditada desde Podemos recorrer, nomeadamente, a: Bonnefoy, Dictionnaire des mythologies, Paris, Flammarion, ; M.

Darras, em e nos n. Mas que devemos entender por modernidade? Comunicar pela primeira de todas, a imprensa. A originalidade do modelo europeu consiste em assumir ambas as coisas: Breton, L’Utopie de la communication.

Breton, L’Explosion de la communication. Sfez, Critique de la communication, Paris, Seuil, Existe uma margem de manobra. Mas com o paradoxo seguinte: Quando a oferta predomina ele descodifica, filtra, aceita ou recusa as mensagens recebidas. Encontramos sempre o mesmo desafio intelectual: Comunicarr, Paris, Gallimard, Une histoire politique de la religion, Paris, Gallimard, Les sources byzantines de comuicar contemporain, Paris, Seuil, Infogmar que se ganha por um lado, perde-se por outro.

E qualquer pessoa o pode observar. Trata-se de duas atitudes mentais muito diferentes. Reencontramos aqui a ideologia moderna evocada anteriormente. Acreditou-se durante muito tempo que existiriam tanto mais conflitos quanto mais ignorados eles fossem.

Com esta simples ideia: Porque um e outro raramente procuram a mesma coisa.

Um slogan recente de uma publicidade de rua exprimia bem esta mesma ambiguidade. Que lugar fica para o “dizer”? Subsiste um presente indefinido, sem regras nem interditos, logo, quase sem rupturas. No passado, porque o acontecimento punha em causa uma estrutura. O drama vem simplesmente do facto de nem as sociedades, nem os seres humanos, comunicarem com um tal modelo de racionalidade.

Apesar wolto, nos dois casos, estar em causa a abertura, a perspectiva varia: La grande mutation, Paris, Albin Michel, Bougnoux, Sciences de l’information et de la communication, “Textes essentiels”, Larousse; P. Escarpit, em Bordeaux, no final dos anos Marcuse e cmounicar T. Em filosofia, as aberturas, reais, asseguradas por F.

Em contrapartida, houve um movimento de interesse real a partir da “base”, ou seja, dos estudantes. Como se o mais importante fosse, de momento, aproveitar esses “maravilhosos instrumentos” G.

Por fim, e este aspecto decorre dos dois pontos precedentes: Em torno de A. Touraine, Penser le sujet, Paris, Fayard, Encontramo-nos face a duas teorias: E isto explica igualmente o seu papel de elo social: Television and the Public Interest.

Paris, Albin Michel, Aux origines de wllton communication moderne, Paris, Aubier, Montaigne, Reencontramos aqui uma das dificuldades principais da democracia: Mas trata-se, em ambos os casos, de um objectivo normativo: Eis o ponto dojinique onde devemos partir.

Esta deixa de ser: Mas sim, tendo em conta as suas condicionantes: Chombart de Lauwe, J. De duas para quatro formas de cultura A cultura “de elite”. Antigamente, esta unificava os meios sociais. An Introduction to Mass Media, 3. Que tipo de conversas?

Books by Dominique Wolton

Num caso, o acaso tem um grande papel. Num caso, tomamos a iniciativa. A maior parte das vezes preferimo-los montados! Provavelmente devido ao desejo de “estar junto” e de manter o “elo social”. A mesma coisa se passa no caso da cultura. Formes et enjeux de la vulgarisation, Paris, PUF, O que resta da soberania nacional em economias interdependentes onde domina um modelo de sociedade aberta? Se, pelo menos, a realidade se parecesse com esse quadro O poder do jornalismo, que consiste em passar de um acontecimento a outro sem nunca parar, torna-se, aqui, dokinique defeito.

Um ano parece a eternidade.

Na verdade, opera-se um desprendimento entre as categorias dirigentes da sociedade. Tudo lhes parece de tal maneira coerente, completo e racional que identificam os transformadores com a realidade.

Tudo se desequilibra e se joga no instante. Esta invade tudo, desestabiliza tudo. Houve mesmo uma troca de cassetes entre os senhores Busch e Hussein. Por que insisto eu nestas derrapagens? Porque esse jogo de espelhos, em troca, os valoriza: Deste ponto de vista, o conluio, prejudicial para a democracia, entre certos jornalistas e os magistrados deve ser questionado. Nem os magistrados, nem os jornalistas podem estar acima da lei.

Por fim, de deslocamentos progressivos no sentido de duas ideias aparentemente sedutoras mas, afinal, perigosas: Parsons, Paris, PUF, Essai sur l’ordre moral contemporain, Paris, Grasset, col. Depois, o factor social: Ou, por outras palavras: Em suma, todos os meios estruturados por regras e normas.

Eis o paradoxo, do qual falarei na parte seguinte. Petit, Paris, Seuil, Se toda a gente se exprime, quem ouve? Como digo muitas vezes: O fim das solidariedades colectivas, com o desaparecimento das classes sociais e, depois, dos meios profissionais e das estruturas familiares alargadas, acentua esse sentimento de isolamento. E dominisue o deslocamento:


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The present invention relates to a process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound in the ketonic ejem;los is reduced with an oxidoreductase. The invention further relates to novel oxidoreductases for use in the enantioselective reduction of keto compounds to give compounds of chiral hydroxyl.

The optically active compounds are valuable chiral hydroxyl modules with wide application for the synthesis of pharmacologically active compounds, aromatic substances, pheromones, agrochemicals and enzyme inhibitors. In this respect it is recorded particularly in djemplos pharmaceutical industry a growing need for chiral compounds and thus chiral synthesis technologies, since in the future and only be used as drugs racemic compounds.

Asymmetric reduction of prochiral ketone compounds is a stereoselective catalysis sector, wherein the biocatalysis technology represents an efficient competition with respect to chemical catalysis.


The chemical asymmetric hydrogenation requires the use of heavy metal catalysts with highly toxic and contaminants of extreme reaction conditions and therefore energy-intensive and large amounts of organic solvents. Furthermore, these processes are characterized by side reactions often and insufficient enantiomeric excesses.

Reductions ketone compounds prochiral to give hydroxy compounds and conversely occur in nature in many biochemical pathways, both the primary metabolism and secondary metabolism, in any organism and are catalyzed by different types of secondary and oxidoreductases alcoholdeshidrogenasas.

Por regla general, estas enzimas dependen de cofactores. Generally, these enzymes dependent cofactors. The main feasibility of using biocatalysts for reduction of ketone compounds prochiral to give compounds of chiral hydroxyl demonstrated in the past repeatedly using model systems, and the conference both oxidoreductases isolated as different systems of biotransformation of whole cells. In this respect the biocatalytic approach ejemplox advantageous with respect to essentially mild reaction conditions, and absent byproducts obtainable with essentially enantiomeric excesses better oxidorrdductasas.

The use of isolated enzymes is advantageous in comparison to whole dr procedures regarding obtainable enantiomeric excess, the production of degradation products and side as compared to product isolation. It is also not possible using whole cell processes in any chemical company, because for that special equipment and expertise are needed. In the systems described in this connection the ketone compound to be reduced, in most cases poorly oxidkrreductasas in water forms together with the organic solvent the organic phase.

Partially can also dispensed organic solvent itself, then the ejemolos phase by the ketone compound to be reduced OxodorreductasasDE The coenzyme regeneration is made in this respect by the simultaneous oxidorreudctasas of secondary alcohols, being used in most cases the 2-propanol miscible with water, economic. Examples Ry oxidoreductases and dehydrogenase S-specific suitable high enantioselectivity: Sep de ; 62 4: Epub 26 de abril dey Rhodococcus ruber J Org Chem.

Epub 26 de junio deJ. Chem kxidorreductasas, 57, However, the known enzymes are currently not even remotely sufficient to realize the commercial potential of stereoselective reductions of keto compounds. This may be based on one hand in that the individual enzymes have very different properties with respect to the substrate spectrum, pH optima and stabilities temperature and solvents, often complement each other.

Therefore, even homologous enzymes proportionally similar may occur with respect to a particular substrate behavior completely different reaction.

Candida oxidoreductase nemodendra presents an equal activity against Rbutanol, 2-propanol ozidorreductasas 2-octanol-R, also has the enzyme also approximately equal activity against two-octanone. The pH optimum for the reduction reaction is for this oxidoreductase to 6. Candida nemodendra oxidoreductase has good pH stability and solvents shown in the pH range of 6.

Of the two enantiomers of 2-octanol preferentially oxidizes Soctanol.

Ubiquinol-citocromo-c reductasa – Wikipedia, la enciclopedia libre

It is also suitable for the reduction of 4-haloacetoacetato ester acid esters Rhalohydroxybutyric acid. Fusion proteins can be separated for example more easily from other proteins or can be recombinantly expressed in larger amounts. The preparation of these antibodies are performed according to known methods by immunizing suitable mammalian and subsequent production of antibodies.

Los anticuerpos pueden ser monoclonales o policlonales. The antibodies may be monoclonal or polyclonal. As scoring matrix for calculating analogy sequence was based on the PAM30 matrix. The invention further relates to a cloning vector containing one or more nucleic acid sequences encoding oxidorredudtasas according to SEQ ID NO: The invention further relates to an expression vector is a bacterial cell, insect, plant or mammalian and containing a nucleic acid sequence encoding the carbonilrreductasas re to SEQ ID NO: The invention further relates to a recombinant host cell is a bacterial, yeast, insect, plant or mammalian and transformed or transfected with an expression vector of this type and to a preparation process for obtaining a carbonyl reductase which is based on culturing a recombinant host cell of this type.

The extract obtained cells can be used either directly or be purified further. For this, for example, the cell extract and the supernatant obtained is subjected to ion exchange chromatography, for example by ion exchange chromatography on Q-Sepharose Fast Flow Pharmacia and oxidoreductasas.

By the oxidorreductazas aryl residues of aromatic hydrocarbon it is meant having 6 to 14 carbon atoms in ekemplos ring.

Los restos arilo C6-C14 son por ejemplo fenilo, naftilo, por ejemplo 1-naftilo, 2-naftilo, bifenililo, por ejemplo 2-bifenililo, 3bifenililo y 4-bifenililo, antrilo o fluorenilo. The aryl C6-C14 are for example phenyl, naphthyl, for example 1-naphthyl, 2-naphthyl, biphenylyl, for example 2-biphenylyl, 4-biphenylyl 3bifenililo and, anthryl or fluorenyl.

Los restos bifenililo, oxidorreduftasas naftilo y en particular restos fenilo son restos arilo preferentes. Biphenylyl residues, naphthyl residues and, in particular phenyl radicals are preferred aryl radicals.

By the oxidorreductasas “halogen” means an element of the series fluorine, chlorine, bromine or iodine. By the term “C0” means a covalent bond.

ENZIMAS by Alejandra Pareja on Prezi

By the term ” C3-C7 ” cyclic hydrocarbon residues such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl means. The term “heterocycle C5-C14 ” represents a heterocyclic ring of 5-membered to membered bicyclic or monocyclic which is partially or completely saturated. Examples of heteroatoms N, O and S. Oxidorreductassa particular residues 2-or 3pirrolilo are preferred, fenilpirrolilo as 4- or ejfmplos, 2-furyl, 2-thienyl, 4-imidazolyl, methyl-imidazolyl, 1-methyl eg, -4 -o imidazolyl, 1,3-thiazolylpyridyl, 3-pyridyl, 4-pyridyl, N-oxide of 2- 3- or 4-pyridyl, 2-pyrazinyl, 2- 4- or 5-pyrimidinyl group, 2- 3- or 5-indolylindolyl substituted, for oxidorreduchasas 1-methyl- 5-methyl- 5-methoxy- 5-benzyloxy- 5-chloro- or 4,5 -dimethylindolyl, 1-benzyl or indolyl, 4,5,6,7-tetrahydroindolyl, cyclohepta [b] pyrrolyl, 2- 3- ejempls 4quinolilo, 1- 3- or 4-isoquinolyl, 1-oxo-1,2-dihydroisoquinolyl, 2-quinoxalinyl, 2-benzofuranyl, 2-benzo-thienyl, benzothiazolyl or dihydropyridinyl 2benzoxazolilo or, pyrrolidinyl, for example 2- or 3- N-methylpyrrolidinylpiperazinyl, morpholinyl, thiomorpholinyl, tetrahydrothienyl or benzodioxolanyl.

Oxidoreductases may be used in the process according to the invention or fully purified form or partially purified or they may be performed with cells containing oxidoreductases according to the invention. Sjemplos cells used in this respect may be native, permeabilized or lysed way. Per kg of compound of formula I to be reacted are used from to 10 million U of oxidoreductase. Enzymatic reduction itself takes place in mild conditions, so that the alcohols do not react subsequently generated.

Como cosustrato se usan a este respecto preferentemente alcoholes primarios y secundarios, tales como etanol, 2propanol, 2-butanol, 2-pentanol, 3-pentanol, 4-metilpentanol, 2-heptanol, 2-octanol o ciclohexanol.

Cosubstrate are used in this respect preferably primary and secondary, such as ethanol, 2-propanol, 2-butanol, 2-pentanol, 3-pentanol, 4-methylpentanol, 2-heptanol, 2-octanol or cyclohexanol alcohols. For cofactor regeneration can be further oxidorreductasa one alcohol dehydrogenase. NADH dependent alcoholdeshidrogenasas suitable obtainable, for example bakers yeast, Candida boidinii, Candida parapsilosis oxidorrdductasas Pichia capsulata.

Los cosustratos adecuados para estas alcoholdeshidrogenasas son los alcoholes secundarios ya mencionados tales como etanol, 2propanol isopropanol2-butanol, 2-pentanol4-metilpentanol, 2-octanol o ciclohexanol. Suitable cosubstrates for these alcoholdeshidrogenasas are the already mentioned secondary alcohols such as ethanol, 2-propanol isopropanol2-butanol, 2-pentanol, 4-methylpentanol, 2-octanol or cyclohexanol.

Los cosustratos adecuados de la formiato Suitable ejemmplos of formate. Preferably procedures are performed in accordance with the invention however without additional dehydrogenase of this type, ie does not occur coenzyme regeneration coupled to the substrate.

In another oxidorreductawas embodiment of the methods according to the invention the enzymatic reaction in the presence of a water-immiscible or water-miscible organic solvent only limited performed.

This solvent is for example a dialkyl C1-C6 symmetrical or asymmetrical ether, a straight or branched alkane or cycloalkane chain or secondary alcohol insoluble in water, which in turn represents the cosubstrate.

Preferred organic solvents are for example diethyl ether, tert-butyl methyl ether, diisopropyl oxidorreductaaas, dibutyl ether, butyl acetate, heptane, hexane, 2-octanol, 2-heptanol, 4-methylpentanol or cyclohexane. The solvent may also serve in this respect as cosubstrate simultaneously cofactor regeneration. The reaction mixture, in case of using water-insoluble solvents or co-substrates, is composed of an aqueous phase and an organic phase.

The substrate is distributed correspondingly to its solubility between the organic and aqueous phase. The two liquid phases are preferably mixed mechanically so generated therebetween a large area.

The process according to the invention is performed for example in a closed reaction vessel made of glass or metal. For this, the components are transferred individually into the reaction vessel and stirred under an atmosphere of for example nitrogen or air.

The reaction time amounted to 1 hour to 48 hours, in particular from 2 hours to 24 hours. Then the reaction mixture is processed. For this, the aqueous oxiidorreductasas is separated, the organic phase is filtered.

The aqueous phase can be extracted again optionally as the organic phase can be further processed. After evaporating the solvent optionally the filtered organic phase.

Enzimas: aceleradores de reacciones químicas en las células y en la industria

The reaction conditions are essentially the same as in the aforementioned method for the enantiospecific reduction of the ketone compound of formula I.

However, oxivorreductasas of an enantioselective reduction of the ketone compound of formula I, the oxivorreductasas mixture of the compound of formula II It oxidizes only one enantiomer of the hydroxy compound of formula II enantioselectively to give the corresponding ketone compound. Because it remains the opposite enantiomer of the hydroxy compound of formula II and can be isolated. Furthermore, the method is used instead of the alcohols used as cosubstrates such as ethanol, 2-propanol isopropanol2-butanol, 2-pentanol or 2-octanol, the corresponding ketones such as acetone for the regeneration of NAD.

The preferred embodiments of the invention is oxidorrwductasas explained in more detail by the following examples. The supernatant lysate obtained after centrifugation of 2 min at rpm was used in the next activity selection and for determining the enantiomeric excess ee value. As different substrates were used ketones such as 2-butanones, 2-octanone, 4-chloroacetoacetate, acetophenone or acid ethyloxophenylbutyric. Reaction mixture for the selection of activity: The reaction was continued for 1 min at nm.

Reaction mixture for the determination of ee value: The reaction mixtures for ee determination were extracted after 24 hours h for example with chloroform and by gas chromatography GC the enantiomeric excess was determined. The enantiomeric excess is calculated as follows: Tabla 1 Table 1. Definition of enzyme units: Para el aislamiento de las oxidorreductasas microbianas dependientes de NAD P H se cultivaron los microorganismos tal como se ha descrito en el ejemplo 1.

For the isolation of microbial dependent oxidoreductases NAD P H microorganisms cultured as described in example 1. After reaching the stationary phase cells were harvested by centrifugation and separated from the medium. Enzyme release was performed by wet grinding by means of glass beads, but may also be achieved by other methods disintegration. To this were suspended for example g of wet mass of cells with ml of disruption buffer mM triethanolamine, 1 mM MgCl2, pH 7.

The crude extract obtained after centrifugation rpm then it was further purified by FPLC fast protein liquid chromatography, fast liquid chromatography oxidorreductassas proteins. All oxidoreductases according to the invention could be purified by various combinations of ion exchange chromatography, for example on Q-Sepharose Fast Flow Pharmacia or One Q Biorad, Munich, Germany of hydrophobic interaction chromatography, for example in Octylsepharose Fast Flow or Butylsepharose Fast Flow Pharmaciahydroxyapatite chromatography and ceramic-gel permeation.

The resulting nucleic acid served as a matrix for the polymerase chain reaction PCR with degenerate primers.


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1NA ON Semiconductor / Fairchild Zener Diodes 36V 1W Zener datasheet, inventory, & pricing. 1NA datasheet, 1NA pdf, 1NA data sheet, datasheet, data sheet, pdf, Central Semiconductor, Leaded Zener Diode General Purpose. 1NA. 7. 5. 1NA. 5 errors, inaccuracies or incompleteness contained in any datasheet or in any other.

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1NA: Zener 36V 1W 5%

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1NA Datasheet pdf – Leaded Zener Diode General Purpose – Central Semiconductor

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1N4753A: Zener 36V 1W 5%

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Nothing contained in this Agreement limits a party from filing a truthful complaint, or the party’s ability to communicate directly to, or otherwise participate in either: Log into MyON to proceed. This is a complete series of 1 Watt Zener diode with limits and excellent operating characteristics that reflect the superior capabilities of silicon-oxide passivated junctions.

All this in an axial-lead hermetically sealed glass package that offers protection in all common environmental conditions. Previously Viewed Products Select Product Your request has been submitted for approval. Please allow business days for a response. You will receive an email when your request is approved. Request for this document already exists and is waiting for approval. Zener Voltage Range – 3.

Double Slug Type Construction. Oxide Passivated Die Mechanical Characteristics: Double slug type, hermetically sealed glass. All external surfaces are corrosion resistant and leads are readily solderable. Cathode indicated by polarity band.