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BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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The cultures were discarded after 15 days. Regardless, Joseph et al.

F9S exhibited the best results regarding the degradation of asphaltenes. Spill Sci technol Bull. Bacterial metabolism is readjusted as culture ages and less complex substrates saturated linear alkanes and low molecular weight aromatic compounds are exhausted, shifting towards the synthesis of enzyme complexes geared towards the degradation of more complex bacteriaas.

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Effectiveness of the natural bacterial flora, bioestimulation and bioaumentation on the bioremediation of a hydrocarbon contaminated Antartic soil. Based on our results, however, together with the existing literature, it is possible to make some inferences. We were unable to find previous mentions in the literature of the presence of these microorganisms in hydrocarbon-contaminated environments.

Depth-resolved quantification of anaerobic toluene degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume. Prentice Degradadors, Iberia; Two of the isolated strains belonged to the Pseudomonas genus. Appl Environ Microbiol ;64 Finer taxonomic classification to the level of species was not pursued since, despite the existence of taxonomic identification schemes for microorganisms from marine ecosystems [12, 15], successfully identifying recently described genera and species of marine bacteria requires the application of molecular methods [16].

The obtained isolates were then subjected to selection in Bushnell-Haas medium supplemented with a heavy crude oil, selecting three strains able to degrade this hydrocarbon mixture within a period petfoleo seven days.


BACTERIAS DEGRADADORAS DE HIDROCARBUROS by Mirelly Katherine Diaz Gamarra on Prezi

Strain F10S1 lowered the concentration of all fractions in comparison with the control. Bacterial community dynamics and hydrocarbon degradation during a field-scale evaluation of bioremediation on a mudflat beach contaminated with buried oil. Taxonomic scheme for the identification of marine bacteria.

Bioremediation potential of terrestrial fuel spills. Isolation and characterization of polycyclic aromatic hydrocarbon-degrading degradaroras associated with the rhizosphere of salt marsh plants.

This decrease in isoprenoids is a telltale sign of effective biodegradation; whereas strain F1FLC exhibits values above those of the abiotic control. These authors attributed such a phenomenon to adaptations of these microorganisms to the chronic contamination of their original habitats.

Description of toluene inhibition of methyl bromide biodegradation in seawater and isolation of a marine toluene oxidizer that degrades methyl bromide. Each strain was assayed in triplicate. Viewed as a whole, the latter possess the necessary genetic information to produce all the enzymes required to completely degrade complex hydrocarbon mixtures in damaged areas [4, 5].

Most research on bioremediation technology has focused therefore on aerobic heterotrophic bacteria, due not only to the taxonomic diversity of hydrocarbon-degrading representatives from this group, but to their ability petro,eo use xenobiotic compounds as carbon source in pure cultures [3].

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Aliphatic hydrocarbons decreased in comparison with those of petroloe abiotic control degradaforas the non-resolved background cycloalkanes, resins and asphaltenes. Measures taken after the Exxon Valdez spill included the addition of nutrients in the form of fertilizers Inipol EAP 22 and Customblerwhich increased the rate of oil removal by three-fold [6].

Effects of rhamnolipid biosurfactants on removal of phenanthrene from soil. These cultures were then further incubated for seven days under the same conditions, at the end of which the subculturing cycle was repeated once more.

Two of these strains were phenotypically identified as sp. Had hydrocarbon composition been determined at that point, we would have most likely found practically identical levels of asphaltenes and resins in crudes treated with the strains under examination and in their controls, significantly decreased levels of saturated hydrocarbons in the former, and only a small drop in the concentration of aromatic hydrocarbons, since the bulk of their degradation takes place after 21 days.


Five strains belonging to the latter group could not be identified with the biochemical tests employed in this study. ABSTRACT The isolation of aerobic marine bacteria able to degrade hydrocarbons represents a promising alternative for the decontamination of oceanic and coastal environments.

In protocol B the samples were stirred at rpm for 5 min, bottling mL from each one afterwards into a sterile mL threaded cap bottle, to which 6 mL of Mesa 30 crude and 0. The increased levels of phenols and phenoxides may be directly related to the accumulation of end compounds produced by the degradation of resins and asphaltenes. Environmental biotechnology techniques have been readily used to remediate ecological disasters caused by large oil spills, such as those caused by the collision of tanker Exxon Valdez with Bligh Reef at Prince William Sound, spilling some 50 tons of crude oil in March [6], and the sinking of Prestigewhich spilled 63 tons of crude and ended up affecting 1 km of French and Spanish coastlines in November [7].

The cultures were examined every 24 h, selecting all strains exhibiting vigorous growth within a seven day period for further study.

Microbial degradation of hydrocarbons in the environment.